EML1 Double Nickase Plasmid (h): sc-406445-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EML1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EML1 Double Nickase Plasmid (h) and EML1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EML1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EML1 Antibody (B-3): sc-390841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EML1 Double Nickase Plasmid (h) | sc-406445-NIC | 20 µg | $410.00 | |||
EML1 Double Nickase Plasmid (h2) | sc-406445-NIC-2 | 20 µg | $410.00 |
EML1 (echinoderm microtubule associated protein like 1) encodes a microtubule-binding protein that contributes to microtubule organization and stability, supporting processes such as mitotic spindle function, intracellular trafficking, and neurite outgrowth. Through its interaction with tubulin polymers and microtubule-associated complexes, EML1 influences cytoskeletal dynamics required for cell division and neuronal migration. Genetic perturbation of EML1 has been linked to neurodevelopmental phenotypes, consistent with a role in cortical development and microtubule-dependent morphogenesis. As a cytoskeleton regulator, EML1 is a useful node for studying how microtubule architecture integrates with cell cycle control and polarity pathways.
EML1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EML1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EML1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EML1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EML1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.