Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EB1 Double Nickase Plasmid (h): sc-401367-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EB1 Double Nickase Plasmid (h) and EB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAPRE1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EB1 Antibody (1A11/4): sc-47704
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EB1 Double Nickase Plasmid (h) | sc-401367-NIC | 20 µg | $410.00 | |||
EB1 Double Nickase Plasmid (h2) | sc-401367-NIC-2 | 20 µg | $410.00 |
MAPRE1 encodes end-binding protein 1 (EB1), a core microtubule plus-end tracking protein that recognizes the GTP-tubulin cap and coordinates microtubule growth and stabilization. EB1 serves as a scaffold for +TIP networks, linking dynamic microtubules to kinetochores and the cell cortex to support spindle assembly, chromosome segregation, and directed cell migration. Through interactions with APC, CLIP proteins, and dynein/dynactin regulators, EB1 integrates microtubule dynamics with mitotic progression and cell polarity pathways. Dysregulated MAPRE1/EB1 function and expression have been associated with altered chromosomal stability and cytoskeletal remodeling observed in cancer-related cell biology studies.
EB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAPRE1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAPRE1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAPRE1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAPRE1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.