E6-AP Antibody (F-1): sc-166688

E6-AP Antibody (F-1) is a mouse monoclonal IgG2a light chain antibody that detects E6-AP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E6-AP, also known as ubiquitin protein ligase E3A (UBE3A), plays a crucial role in the ubiquitin-mediated proteolytic pathway, which is essential for regulating protein degradation via the 26S proteasome. This process is vital for maintaining cellular homeostasis and controlling various cellular functions, including the cell cycle and apoptosis. E6-AP facilitates the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to target proteins, a mechanism that is particularly important for the degradation of key regulatory proteins such as the tumor suppressor p53. The interaction between E6-AP and p53 is significant because this interaction not only regulates p53 levels but also influences cellular responses to stress and DNA damage. Disruptions in E6-AP function, often due to genetic mutations, can lead to the accumulation of p53 in the cytoplasm, contributing to the pathogenesis of Angelman syndrome, a neurodevelopmental disorder characterized by severe motor dysfunction and cognitive impairment. Anti-E6-AP antibody (F-1) is therefore an invaluable tool for researchers studying the intricate mechanisms of protein regulation and the implications of E6-AP in various diseases.

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E6-AP Antibody (F-1) References:

1. Degradation of hDlg and MAGIs by human papillomavirus E6 is E6-AP-independent.  |  Grm, HS. and Banks, L. 2004. J Gen Virol. 85: 2815-2819. PMID: 15448342
2. The role of the ubiquitin ligase E6-AP in human papillomavirus E6-mediated degradation of PDZ domain-containing proteins.  |  Kuballa, P., et al. 2007. J Biol Chem. 282: 65-71. PMID: 17085449
3. Ubiquitin-dependent proteolysis of trihydrophobin 1 (TH1) by the human papilloma virus E6-associated protein (E6-AP).  |  Yang, Y., et al. 2007. J Cell Biochem. 101: 167-80. PMID: 17131388
4. The ubiquitin ligase E6-AP is induced and recruited to aggresomes in response to proteasome inhibition and may be involved in the ubiquitination of Hsp70-bound misfolded proteins.  |  Mishra, A., et al. 2009. J Biol Chem. 284: 10537-45. PMID: 19233847
5. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome.  |  Zaaroor-Regev, D., et al. 2010. Proc Natl Acad Sci U S A. 107: 6788-93. PMID: 20351251
6. Post-translational control of IL-1β via the human papillomavirus type 16 E6 oncoprotein: a novel mechanism of innate immune escape mediated by the E3-ubiquitin ligase E6-AP and p53.  |  Niebler, M., et al. 2013. PLoS Pathog. 9: e1003536. PMID: 23935506
7. Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.  |  Huibregtse, JM., et al. 1993. Mol Cell Biol. 13: 775-84. PMID: 8380895
8. The human E6-AP gene (UBE3A) encodes three potential protein isoforms generated by differential splicing.  |  Yamamoto, Y., et al. 1997. Genomics. 41: 263-6. PMID: 9143503
9. The ubiquitin-protein ligase E6-associated protein (E6-AP) serves as its own substrate.  |  Nuber, U., et al. 1998. Eur J Biochem. 254: 643-9. PMID: 9688277
10. E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box.  |  Kühne, C. and Banks, L. 1998. J Biol Chem. 273: 34302-9. PMID: 9852095

The preferred E6-AP antibody is E6-AP Antibody (E-4): sc-166689.