Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DSC1 Double Nickase Plasmid (h): sc-402944-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DSC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DSC1 Double Nickase Plasmid (h) and DSC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DSC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DSC1 Antibody (A-4): sc-398590
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DSC1 Double Nickase Plasmid (h) | sc-402944-NIC | 20 µg | $410.00 | |||
DSC1 Double Nickase Plasmid (h2) | sc-402944-NIC-2 | 20 µg | $410.00 |
Human DSC1 encodes desmocollin-1, a calcium-dependent cadherin family adhesion molecule localized to desmosomes in stratified epithelia. DSC1 cooperates with desmogleins and plakoglobin/plakophilins to anchor intermediate filaments and maintain epidermal barrier integrity, contributing to tissue mechanics and differentiation programs. By regulating intercellular junction assembly and epithelial homeostasis, DSC1 is relevant to studies of skin development, barrier dysfunction, and junctional remodeling. Altered desmosomal adhesion and desmocollin expression patterns are frequently examined in contexts of inflammatory skin phenotypes and epithelial dysregulation associated with tumor progression and metastasis.
DSC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DSC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DSC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DSC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DSC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.