DRIM CRISPR/Cas9 KO Plasmid (h): sc-409900
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DRIM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DRIM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DRIM CRISPR/Cas9 KO Plasmid (h) | sc-409900 | 20 µg | $397.00 |
Overview
UTP20 (also known as DRIM) encodes a nucleolar factor required for ribosome biogenesis, functioning in the maturation of pre-rRNA and assembly of the 60S large ribosomal subunit. By supporting nucleolar homeostasis and efficient ribosome production, DRIM contributes to global translational capacity and proteostasis, processes tightly linked to cell growth and stress adaptation. Disruption of ribosome biogenesis can engage nucleolar stress signaling and p53-dependent checkpoints, connecting DRIM-associated pathways to proliferation control. Altered regulation of ribosome production is frequently observed in cancer and other disorders of cellular growth, making DRIM a useful node for mechanistic studies of translational regulation and stress responses.
DRIM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UTP20 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UTP20 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UTP20 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DRIM protein expression.
This CRISPR knockout system enables efficient generation of UTP20-deficient cell models for investigation of DRIM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UTP20 exon(s) critical for DRIM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UTP20 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DRIM CRISPR/Cas9 KO Plasmid (h) and DRIM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UTP20 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DRIM HDR Plasmid (h) and DRIM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UTP20 homology arms to support homology-directed repair at defined UTP20 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.