DNase II Double Nickase Plasmid (h): sc-405046-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNase II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DNase II Double Nickase Plasmid (h) and DNase II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNASE2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNase II Double Nickase Plasmid (h) | sc-405046-NIC | 20 µg | $410.00 | |||
DNase II Double Nickase Plasmid (h2) | sc-405046-NIC-2 | 20 µg | $410.00 |
Human DNASE2 encodes DNase II, an acidic endonuclease localized primarily to lysosomes that catalyzes degradation of DNA within phagolysosomes and autolysosomes. This activity supports clearance of apoptotic cell–derived nucleic acids and genomic DNA from engulfed material, helping maintain nucleic acid homeostasis during phagocytosis and autophagy. By limiting persistence of intracellular DNA, DNase II influences innate immune sensing pathways linked to nucleic acid recognition and downstream inflammatory signaling. Dysregulated DNASE2 function has been associated with aberrant accumulation of DNA and immune activation phenotypes, making it relevant to studies of inflammation, autoimmunity, and macrophage biology.
DNase II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNASE2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNASE2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNASE2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNASE2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.