Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DMRT1 Double Nickase Plasmid (h): sc-402856-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DMRT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DMRT1 Double Nickase Plasmid (h) and DMRT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DMRT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DMRT1 Antibody (A-9): sc-377167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DMRT1 Double Nickase Plasmid (h) | sc-402856-NIC | 20 µg | $410.00 | |||
DMRT1 Double Nickase Plasmid (h2) | sc-402856-NIC-2 | 20 µg | $410.00 |
DMRT1 encodes a DM-domain transcription factor that binds sequence-specific DNA elements to regulate sex determination and differentiation programs, with prominent roles in Sertoli and germ cell development. In human tissues it participates in transcriptional networks controlling gonadal fate, meiosis, and maintenance of testicular identity through coordinated regulation of downstream developmental genes. Altered DMRT1 dosage or dysregulated expression is associated with disorders of sex development and impaired spermatogenesis, reflecting its sensitivity to gene regulatory balance during gonadogenesis. Because it is a lineage-defining regulator, DMRT1 is frequently studied in models of reproductive development, cell fate decisions, and transcriptional control.
DMRT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DMRT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DMRT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DMRT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DMRT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.