Date published: 2026-11-6

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Dhh Antibody (A-10): sc-133243

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Datasheets
  • Dhh Antibody (A-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 227-311 of Dhh of human origin
  • recommended for detection of Dhh of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Dhh Antibody (A-10) for WB applications. These reagents are now offered in bundles with Dhh Antibody (A-10) (see ordering information below).
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    Dhh Antibody (A-10) is a mouse monoclonal IgG2a antibody that detects Desert hedgehog (Dhh) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Desert hedgehog (Dhh) plays a crucial role in developmental processes, particularly in regulating cell differentiation and tissue patterning during embryogenesis. Dhh (A-10) antibody targets a secreted protein that interacts with specific receptors on target cells, initiating signaling pathways vital for proper organ development and maintenance. Desert hedgehog (Dhh) is involved in male reproductive system formation and has been implicated in various cancers, making anti-Dhh antibody (A-10) an important tool for research in developmental biology and oncology. Dhh monoclonal antibody (A-10) provides reliable detection of this key protein, facilitating studies that explore its function and interactions in both normal and pathological contexts.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

    Download thiomersal SDS

    Dhh Antibody (A-10) References:

    1. Role of DHH superfamily proteins in nucleic acids metabolism and stress tolerance in prokaryotes and eukaryotes.  |  Srivastav, R., et al. 2019. Int J Biol Macromol. 127: 66-75. PMID: 30578903
    2. GATA4/6 regulate DHH transcription in rat adrenocortical autografts.  |  Yoshida, T., et al. 2020. Sci Rep. 10: 446. PMID: 31949236
    3. Full-length Dhh and N-terminal Shh act as competitive antagonists to regulate angiogenesis and vascular permeability.  |  Hollier, PL., et al. 2021. Cardiovasc Res. 117: 2489-2501. PMID: 33063110
    4. Dhh signaling pathway regulates reconstruction of seminiferous tubule-like structure.  |  Min, M., et al. 2022. Reprod Biol. 22: 100684. PMID: 35987158
    5. Hedgehog Signaling in Gonadal Development and Function.  |  Dilower, I., et al. 2023. Cells. 12: PMID: 36766700
    6. Mineralocorticoid receptor knockout in Schwann cells alters myelin sheath thickness.  |  González-Mayoral, A., et al. 2023. J Endocrinol. 258: PMID: 37195271
    7. Nav1.7 is essential for nociceptor action potentials in the mouse in a manner independent of endogenous opioids.  |  Deng, L., et al. 2023. Neuron. 111: 2642-2659.e13. PMID: 37352856
    8. Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration.  |  Norris, AM., et al. 2023. Nat Commun. 14: 3766. PMID: 37355632
    9. Neuroimaging in PRUNE1 syndrome: a mini-review of the literature.  |  Scorrano, G., et al. 2023. Front Neurol. 14: 1301147. PMID: 38178891
    10. Short-term outcomes of treatment switch to faricimab in patients with aflibercept-resistant neovascular age-related macular degeneration.  |  Schneider, M., et al. 2024. Graefes Arch Clin Exp Ophthalmol. 262: 2153-2162. PMID: 38416237

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Dhh Antibody (A-10)

    sc-133243
    200 µg/ml
    $322.00

    Dhh Antibody (A-10): m-IgG Fc BP-HRP Bundle

    sc-539874
    200 µg Ab; 10 µg BP
    $361.00

    Dhh Antibody (A-10): m-IgG2a BP-HRP Bundle

    sc-546805
    200 µg Ab; 10 µg BP
    $361.00

    What is the recommended starting dilution for Western blot for Dhh (A-10): sc-133243 monoclonal antibody?

    Asked by: DefinitelyNotMatt
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-133243.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by QZ from Nice Western blot data of Dhh expression in nonNice Western blot data of Dhh expression in non-transfected and human Dhh transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-02-12
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    Dhh Antibody (A-10) is rated 5.0 out of 5 by 1.
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