Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DGS8 Double Nickase Plasmid (h): sc-404631-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGS8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DGS8 Double Nickase Plasmid (h) and DGS8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DGCR8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DGS8 Antibody (E-10): sc-377249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGS8 Double Nickase Plasmid (h) | sc-404631-NIC | 20 µg | $410.00 | |||
DGS8 Double Nickase Plasmid (h2) | sc-404631-NIC-2 | 20 µg | $410.00 |
DGCR8 (DGS8) encodes a core component of the nuclear Microprocessor complex that partners with DROSHA to recognize and cleave primary microRNA transcripts into precursor miRNAs, shaping global miRNA biogenesis. Through this role, DGCR8 influences post-transcriptional gene regulation programs that control cell cycle progression, differentiation, and stress responses. DGCR8-dependent miRNA pathways intersect with chromatin regulation and signaling networks that tune lineage commitment and RNA homeostasis. Altered DGCR8 dosage or function has been associated with dysregulated miRNA profiles and is studied in the context of neurodevelopmental and proliferative disease mechanisms.
DGS8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DGCR8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DGCR8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DGCR8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DGCR8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.