Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DGK-ι CRISPR/Cas9 KO Plasmid (h): sc-406306
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGK-ι CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DGK-ι genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DGK-ι Antibody (G-3): sc-166439
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGK-ι CRISPR/Cas9 KO Plasmid (h) | sc-406306 | 20 µg | $397.00 |
Overview
DGKI encodes diacylglycerol kinase iota (DGK-ι), an enzyme that phosphorylates diacylglycerol (DAG) to generate phosphatidic acid, thereby shaping the amplitude and duration of lipid second-messenger signaling. By limiting DAG availability, DGK-ι modulates downstream DAG-responsive effectors such as protein kinase C and RasGRP, influencing pathways that govern membrane trafficking, cytoskeletal dynamics, and stimulus-dependent transcriptional programs. DGK-ι activity is linked to regulation of cell signaling networks that coordinate proliferation, differentiation, and migration across multiple cell types. Dysregulated DAG/phosphatidic acid balance and DGK-family signaling have been investigated in contexts including cancer biology, immune signaling, and neurological processes.
DGK-ι CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DGKI gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DGKI together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DGKI open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DGK-ι protein expression.
This CRISPR knockout system enables efficient generation of DGKI-deficient cell models for investigation of DGK-ι signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DGKI exon(s) critical for DGK-ι function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DGKI genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DGK-ι CRISPR/Cas9 KO Plasmid (h) and DGK-ι CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DGKI locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DGK-ι HDR Plasmid (h) and DGK-ι HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DGKI homology arms to support homology-directed repair at defined DGKI target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.