DDX37 CRISPR/Cas9 KO Plasmid (h): sc-412866
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX37 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX37 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX37 CRISPR/Cas9 KO Plasmid (h) | sc-412866 | 20 µg | $397.00 |
Overview
DHX37 encodes the human ATP-dependent RNA helicase DDX37, a DEAD-box family protein implicated in ribosome biogenesis and RNA metabolism. DDX37 participates in nucleolar processes including pre-rRNA processing and maturation of the small ribosomal subunit, supporting efficient translation and cell-cycle progression. Through its roles in RNA helicase–driven remodeling of ribonucleoprotein complexes, DDX37 is positioned within pathways that coordinate RNA processing with proliferative programs. Genetic disruption or dysregulation of DHX37 has been associated with developmental and reproductive phenotypes, making it relevant for studying tissue-specific gene regulation and disease mechanisms linked to impaired RNA processing.
DDX37 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DHX37 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DHX37 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DHX37 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX37 protein expression.
This CRISPR knockout system enables efficient generation of DHX37-deficient cell models for investigation of DDX37 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DHX37 exon(s) critical for DDX37 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DHX37 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX37 CRISPR/Cas9 KO Plasmid (h) and DDX37 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DHX37 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX37 HDR Plasmid (h) and DDX37 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DHX37 homology arms to support homology-directed repair at defined DHX37 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.