Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDX33 Double Nickase Plasmid (m): sc-432143-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX33 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX33 Double Nickase Plasmid (m) and DDX33 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dhx33. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX33 Antibody (B-4): sc-390573
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX33 Double Nickase Plasmid (m) | sc-432143-NIC | 20 µg | $410.00 | |||
DDX33 Double Nickase Plasmid (m2) | sc-432143-NIC-2 | 20 µg | $410.00 |
Mouse Dhx33 encodes DDX33, a DEAD-box RNA helicase implicated in ATP-dependent remodeling of RNA–protein complexes that support RNA metabolism and translation. DDX33 has been linked to ribosome biogenesis and nucleolar function, influencing rRNA processing and protein synthesis capacity during cell growth and stress adaptation. Through these activities, DDX33 intersects with pathways controlling proliferation and innate immune signaling outputs that depend on regulated RNA processing. Dysregulation of RNA helicase function is broadly relevant to oncogenic programs and inflammatory phenotypes, making Dhx33 a useful node for mechanistic studies of RNA-centric control of cell state.
DDX33 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dhx33 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dhx33. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dhx33 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dhx33-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.