DDR2 Double Nickase Plasmid (h): sc-400414-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDR2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDR2 Double Nickase Plasmid (h) and DDR2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDR2 Antibody (3B11E4): sc-81707
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDR2 Double Nickase Plasmid (h) | sc-400414-NIC | 20 µg | $410.00 | |||
DDR2 Double Nickase Plasmid (h2) | sc-400414-NIC-2 | 20 µg | $410.00 |
Discoidin domain receptor 2 (DDR2) is a collagen-activated receptor tyrosine kinase that couples extracellular matrix sensing to intracellular signaling controlling cell adhesion, migration, proliferation, and matrix remodeling. Upon binding fibrillar collagens, DDR2 undergoes autophosphorylation and engages pathways such as MAPK/ERK, PI3K–AKT, SRC family signaling, and cytoskeletal regulators, influencing epithelial–mesenchymal transitions and stromal interactions. DDR2 activity is closely linked to connective tissue homeostasis and collagen turnover, and altered DDR2 signaling has been associated with fibrosis-related remodeling and tumor microenvironment dynamics. As a human DDR2 target, it is frequently studied in models of extracellular matrix–driven signaling, invasive behavior, and receptor tyrosine kinase cross-talk.
DDR2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.