Dcun1D1 Double Nickase Plasmid (h): sc-405179-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dcun1D1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dcun1D1 Double Nickase Plasmid (h) and Dcun1D1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCUN1D1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dcun1D1 Antibody (3E1): sc-81835
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dcun1D1 Double Nickase Plasmid (h) | sc-405179-NIC | 20 µg | $410.00 | |||
Dcun1D1 Double Nickase Plasmid (h2) | sc-405179-NIC-2 | 20 µg | $410.00 |
DCUN1D1 encodes Dcun1D1, an accessory factor for cullin-RING E3 ubiquitin ligases that promotes neddylation of cullin proteins and thereby enhances ubiquitin-dependent proteostasis. Through regulation of cullin activation, Dcun1D1 influences cell-cycle progression, DNA damage responses, and signal-dependent turnover of key regulatory proteins. Altered DCUN1D1 expression or amplification has been linked to dysregulated growth programs and has been reported in multiple tumor contexts, supporting its use as a molecular node for studying oncogenic ubiquitin-like modification pathways. Research on DCUN1D1 helps clarify how NEDD8 conjugation interfaces with ubiquitination to control protein stability and cellular fitness under stress.
Dcun1D1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCUN1D1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCUN1D1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCUN1D1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCUN1D1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.