Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DcpS CRISPR/Cas9 KO Plasmid (h): sc-407308
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DcpS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DcpS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DcpS Antibody (A-12): sc-393226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DcpS CRISPR/Cas9 KO Plasmid (h) | sc-407308 | 20 µg | $397.00 |
Overview
DCPS encodes the human DcpS scavenger decapping enzyme, a histidine triad (HIT) hydrolase that cleaves residual cap structures generated during 3′→5′ mRNA decay, recycling cap-derived metabolites and helping maintain RNA turnover homeostasis. By hydrolyzing cap dinucleotides such as m7GpppN, DcpS complements cytoplasmic and nuclear RNA quality-control processes and supports efficient coupling between mRNA degradation and nucleotide salvage. Altered DCPS activity can perturb transcriptome stability and RNA surveillance, linking this pathway to phenotypes associated with dysregulated gene expression programs. Consequently, DCPS is frequently studied in the context of RNA metabolism, stress-adaptive transcriptional responses, and mechanisms that shape mRNA decay kinetics.
DcpS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCPS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCPS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCPS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DcpS protein expression.
This CRISPR knockout system enables efficient generation of DCPS-deficient cell models for investigation of DcpS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCPS exon(s) critical for DcpS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCPS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DcpS CRISPR/Cas9 KO Plasmid (h) and DcpS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCPS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DcpS HDR Plasmid (h) and DcpS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCPS homology arms to support homology-directed repair at defined DCPS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.