D2DR/Dopamine D2 Receptor Antibody (B-10): sc-5303

D2DR Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects D2DR of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-D2DR antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. D2DR, a member of the G protein-coupled receptor family, plays a crucial role in the central nervous system by mediating the effects of dopamine, a key neurotransmitter involved in regulating mood, reward, and motor control. D2DR is primarily located in the brain, particularly in areas such as the striatum and the pituitary gland, where D2DR is essential for modulating neurotransmission and influencing various physiological processes. D2DR signaling has been implicated in several neuropsychiatric disorders, including schizophrenia and Parkinson′s disease, as proper functioning is vital. Understanding the localization and function of D2DR is important for developing targeted therapies that can effectively address these conditions.

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D2DR/Dopamine D2 Receptor Antibody (B-10) References:

1. Structural subtypes of the dopamine D2 receptor are functionally distinct: expression of the cloned D2A and D2B subtypes in a heterologous cell line.  |  Hayes, G., et al. 1992. Mol Endocrinol. 6: 920-6. PMID: 1323056
2. Two kinases mediate agonist-dependent phosphorylation and desensitization of the beta 2-adrenergic receptor.  |  Hausdorff, WP., et al. 1990. Symp Soc Exp Biol. 44: 225-40. PMID: 1983369
3. Multiple second messenger pathways of alpha-adrenergic receptor subtypes expressed in eukaryotic cells.  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
4. Targeting dopamine receptors subtype 2 (D2DR) in pheochromocytomas: head-to-head comparison between in vitro and in vivo findings.  |  Saveanu, A., et al. 2013. J Clin Endocrinol Metab. 98: E1951-5. PMID: 24009136
5. Selective blockade of spinal D2DR by levo-corydalmine attenuates morphine tolerance via suppressing PI3K/Akt-MAPK signaling in a MOR-dependent manner.  |  Dai, WL., et al. 2018. Exp Mol Med. 50: 1-12. PMID: 30429454
6. The dopamine D1-D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury.  |  Bao, YN., et al. 2021. Exp Mol Med. 53: 235-249. PMID: 33558591
7. A novel dopamine D2 receptor-NR2B protein complex might contribute to morphine use disorders.  |  Li, A., et al. 2023. Eur J Pharmacol. 961: 176174. PMID: 37939993
8. Agonist-induced desensitization of dopamine D1 receptor-stimulated adenylyl cyclase activity is temporally and biochemically separated from D1 receptor internalization.  |  Ng, GY., et al. 1995. Proc Natl Acad Sci U S A. 92: 10157-61. PMID: 7479745
9. The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
10. Molecular and chemical neuropharmacology of dopamine receptor subtypes.  |  Ogawa, N. 1995. Acta Med Okayama. 49: 1-11. PMID: 7762403
11. S311C D2DR variant: no association with schizophrenia.  |  Sobell, J., et al. 1994. Lancet. 344: 621-2. PMID: 7914994
12. The D2 dopamine receptor isoforms signal through distinct Gi alpha proteins to inhibit adenylyl cyclase. A study with site-directed mutant Gi alpha proteins.  |  Senogles, SE. 1994. J Biol Chem. 269: 23120-7. PMID: 7916015

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-D2DR/Dopamine D2 Receptor Antibody (B-10) has 169 citations in a variety of scientific publications.

Primary antibodies like Anti-D2DR/Dopamine D2 Receptor Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

D2DR/Dopamine D2 Receptor Antibody (B-10) Specifications

Alternate Product Name: DRD2

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-50

Epitope Target: D2DR

Epitope Species: human

Product Reactivity/Detection: D2DR long and short forms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 169 citations in various scientific publications

Available Conjugates/Forms for D2DR/Dopamine D2 Receptor Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin