CyPA Antikörper (6-YD13) Empfohlen für die Detektion von CyPA aus der Spezies mouse, rat und human per WB, IP und ELISA

CyPA Antikörper (6-YD13): sc-134310

CyPA Antikörper (6-YD13) wurde bewertet mit 4.2 von 5 von 5.
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Datenblatt

    • Anti-CyPA Antikörper 6-YD13 ist ein monoklonales IgG2a κ CyPA Antikörper, verwendet in 6 wissenschaftlichen Veröffentlichungen, in einer Menge von 100 µg/ml
    • gezogen gegen Volllänge-CyPA aus der Spezies human
    • Empfohlen für die Detektion von CyPA aus der Spezies mouse, rat und human per WB, IP und ELISA
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom CyPA (6-YD13): sc-134310 zu erhalten.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Gefragt von: jerojero
Thank you for your question We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Beantwortet von: Santa Cruz TS
Veröffentlichungsdatum: 2017-01-27
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Rated 1 von 5 von aus Not nearly as good as their old rabbit polyclonal! I did an IP to pull down CypA (using abcam's antibody as its better), and then came back with this 6-YD13 mouse monoclonal antibody (right hand blot on image). This is in HEK cells. The antibody band for CypA is very weak signal and the antibody bands for the light chain and heavy chain from the IP beads is very overpowering- I never saw this with the old CypA antibody. I thought that maybe the IP extract is degraded (unlikely though as I've done this lots of times before using my protocol). So I reprobed the same blot with the old C-14 polyclonal rabbit antibody I had left over and the bands for CypA are clear as day . Both antibodies were used at 1:1000 diluted in 5% BSA PBST. I can't increase the amount of 6-YD13 used as it will also increase the signal of the light/heavy chain and will bleach the CypA signal more.
Veröffentlichungsdatum: 2017-07-17
Rated 5 von 5 von aus Strong signal in WB Very clear band in WB at 1:500 dilution. Several publications available for WB and IF in mouse and human samples.
Veröffentlichungsdatum: 2016-12-21
Rated 5 von 5 von aus powerful tool Made outstanding contribution in my work and paper。
Veröffentlichungsdatum: 2016-11-03
Rated 5 von 5 von aus Robust band in western blot analysis of CyPA Robust band in western blot analysis of CyPA expression in K-562 whole cell lysate. -SCBT QC
Veröffentlichungsdatum: 2015-03-11
Rated 5 von 5 von aus Strong band present in western blot using Strong band present in western blot using Jurkat whole cell lysate. -SCBT QC
Veröffentlichungsdatum: 2013-10-04
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