cyclin T1 Antibody (E-7): sc-398695

Cyclin T1 Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects cyclin T1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cyclin T1 plays a crucial role in transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for phosphorylating the C-terminal domain of RNA polymerase II. This phosphorylation enables RNA polymerase II to transition from transcription initiation to elongation, facilitating mRNA production. The interaction between cyclin T1 and HIV-1 Tat protein enhances Tat′s ability to bind viral TAR RNA, promoting recruitment of the cyclin T1/Cdk9 complex to RNA polymerase II. This mechanism underscores cyclin T1′s importance in normal cellular transcription processes and viral transcription, highlighting potential therapeutic intervention in HIV. Anti-cyclin T1 antibody (E-7) serves as an invaluable tool for researchers studying transcription regulation and viral interactions, providing reliable detection and characterization of cyclin T1.

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cyclin T1 Antibody (E-7) References:

1. Pattern of expression of cyclin T1 in human tissues.  |  De Luca, A., et al. 2001. J Histochem Cytochem. 49: 685-92. PMID: 11373315
2. Functional interaction between cyclin T1/cdk9 and Puralpha determines the level of TNFalpha promoter activation by Tat in glial cells.  |  Darbinian, N., et al. 2001. J Neuroimmunol. 121: 3-11. PMID: 11730934
3. Recruitment of human cyclin T1 to nuclear bodies through direct interaction with the PML protein.  |  Marcello, A., et al. 2003. EMBO J. 22: 2156-66. PMID: 12727882
4. Cdk9/Cyclin T1 complex: a key player during the activation/differentiation process of normal lymphoid B cells.  |  De Falco, G., et al. 2008. J Cell Physiol. 215: 276-82. PMID: 18205180
5. Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.  |  Anand, K., et al. 2008. Nat Struct Mol Biol. 15: 1287-92. PMID: 19029897
6. Functional characterization of a human cyclin T1 mutant reveals a different binding surface for Tat and HEXIM1.  |  Kuzmina, A., et al. 2012. Virology. 426: 152-61. PMID: 22342181
7. A Cyclin T1 point mutation that abolishes positive transcription elongation factor (P-TEFb) binding to Hexim1 and HIV tat.  |  Verstraete, N., et al. 2014. Retrovirology. 11: 50. PMID: 24985203
8. Increased cyclin T1 expression as a favorable prognostic factor in treating gastric adenocarcinoma.  |  Jin, JS., et al. 2015. Oncol Lett. 10: 3712-3718. PMID: 26788195
9. Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells.  |  Couturier, J., et al. 2019. Virol J. 16: 22. PMID: 30786885
10. A Truncated Isoform of Cyclin T1 Could Contribute to the Non-Permissive HIV-1 Phenotype of U937 Promonocytic Cells.  |  Alberio, T., et al. 2024. Viruses. 16: PMID: 39205150

The preferred cyclin T1 antibody is cyclin T1 Antibody (E-3): sc-271348.