Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cyclin D3 Double Nickase Plasmid (h): sc-400634-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin D3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cyclin D3 Double Nickase Plasmid (h) and cyclin D3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCND3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin D3 Antibody (D-7): sc-6283
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin D3 Double Nickase Plasmid (h) | sc-400634-NIC | 20 µg | $410.00 | |||
cyclin D3 Double Nickase Plasmid (h2) | sc-400634-NIC-2 | 20 µg | $410.00 |
CCND3 encodes cyclin D3, a regulatory cyclin that partners with CDK4/6 to phosphorylate RB family proteins and promote G1/S cell-cycle progression. By integrating mitogenic signaling with transcriptional programs, cyclin D3 contributes to checkpoint control, proliferation, and lineage-specific differentiation, particularly in hematopoietic contexts. Dysregulated CCND3 activity is linked to aberrant cell-cycle entry and genomic instability observed across multiple malignancy-associated models, making it a common node in studies of cell growth control and oncogenic signaling pathways.
cyclin D3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCND3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCND3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCND3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCND3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.