cyclin A Antibody (AT10.2): sc-53227
- cyclin A Antibody (AT10.2) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
- raised against human recombinant cyclin A
- recommended for detection of cyclin A of mouse, rat and human origin by WB, IP, IF and IHC(P)
- See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for cyclin A Antibody (AT10.2) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin A Antibody (AT10.2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Cyclin A Antibody (AT10.2) is a mouse monoclonal IgG1 antibody that detects cyclin A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-cyclin A antibody (AT10.2) plays a pivotal role in studying the regulation of the eukaryotic cell cycle, particularly during the S phase, where cyclin A is crucial for the proper progression and timing of DNA replication. Cyclin A is primarily located in the nucleus, where cyclin A associates with cyclin-dependent kinase-2 (Cdk2) to form active complexes that drive the cell cycle forward. Precise regulation of cyclin A is vital, as dysregulation can lead to uncontrolled cell proliferation and has been implicated in various cancers. Cyclin A interacts with several key proteins, including the retinoblastoma protein and transcription factors such as E2F, which are essential for cell cycle control and gene expression. Cyclin A monoclonal antibody (AT10.2) is an invaluable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cell proliferation and transformation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin A Antibody (AT10.2) References:
- Cyclin A transcriptional suppression is the major mechanism mediating homocysteine-induced endothelial cell growth inhibition. | Wang, H., et al. 2002. Blood. 99: 939-45. PMID: 11806997
- Cyclin B and cyclin A confer different substrate recognition properties on CDK2. | Brown, NR., et al. 2007. Cell Cycle. 6: 1350-9. PMID: 17495531
- Regulation of Cyclin A protein in meiosis and early embryogenesis. | Vardy, L., et al. 2009. Proc Natl Acad Sci U S A. 106: 1838-43. PMID: 19181861
- Cyclin A-Cdk1 regulates the origin firing program in mammalian cells. | Katsuno, Y., et al. 2009. Proc Natl Acad Sci U S A. 106: 3184-9. PMID: 19221029
- Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding. | Pascreau, G., et al. 2010. Proc Natl Acad Sci U S A. 107: 2932-7. PMID: 20133761
- The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate centrosome reduplication. | Ferguson, RL., et al. 2010. J Cell Sci. 123: 2743-9. PMID: 20663915
- Expression of cyclin A in A549 cell line after treatment with arsenic trioxide. | Żuryń, A., et al. 2015. Postepy Hig Med Dosw (Online). 69: 1259-67. PMID: 26671917
- Cyclin A2/E1 activation defines a hepatocellular carcinoma subclass with a rearrangement signature of replication stress. | Bayard, Q., et al. 2018. Nat Commun. 9: 5235. PMID: 30531861
- Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division. | Clemm von Hohenberg, K., et al. 2022. Nat Commun. 13: 668. PMID: 35115540
- Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | Zhang, J., et al. 2022. Nat Commun. 13: 2835. PMID: 35595767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin A Antibody (AT10.2) | sc-53227 | 200 µg/ml | $322.00 | |||
cyclin A Antibody (AT10.2): m-IgGκ BP-HRP Bundle | sc-551069 | 200 µg Ab; 40 µg BP | $361.00 |