cSHMT Antibody (A-2): sc-365203
- cSHMT Antibody (A-2) is a mouse monoclonal IgG1 κ cSHMT antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 171-201 near the C-terminus of cSHMT of human origin
- cSHMT Antibody (A-2) is recommended for detection of cSHMT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-cSHMT Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cSHMT Antibody (A-2) for WB and IHC(P) applications. These reagents are now offered in bundles with cSHMT Antibody (A-2) (see ordering information below).
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cSHMT Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects cSHMT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-cSHMT antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cSHMT protein, or cytosolic serine hydroxymethyltransferase, plays a crucial role in cellular metabolism by catalyzing the reversible conversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate, primarily in the cytoplasm and mitochondria. This enzymatic activity is vital for the synthesis of nucleotides, particularly thymidylate, which is essential for DNA replication and repair. Additionally, cSHMT is involved in the regulation of one-carbon metabolism, influencing the availability of methylenetetrahydrofolate for S-adenosylmethionine synthesis, a key methyl donor in numerous biological processes. cSHMT′s tetrameric structure is stabilized by specific residues, such as Lys-71, Arg-80, and Asp-89, which are critical for maintaining catalytic function. The human cSHMT gene is located on chromosome 17p11.2, while the mitochondrial variant is found on chromosome 12q13, highlighting the evolutionary conservation and significance of this enzyme across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cSHMT Antibody (A-2) References:
- Lack of catalytic activity of a murine mRNA cytoplasmic serine hydroxymethyltransferase splice variant: evidence against alternative splicing as a regulatory mechanism. | Liu, X., et al. 2001. Biochemistry. 40: 4932-9. PMID: 11305908
- Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism. | Trivedi, V., et al. 2002. J Biol Chem. 277: 17161-9. PMID: 11877399
- Cytoplasmic serine hydroxymethyltransferase mediates competition between folate-dependent deoxyribonucleotide and S-adenosylmethionine biosyntheses. | Herbig, K., et al. 2002. J Biol Chem. 277: 38381-9. PMID: 12161434
- Identification of amino acid residues, essential for maintaining the tetrameric structure of sheep liver cytosolic serine hydroxymethyltransferase, by targeted mutagenesis. | Jala, VR., et al. 2003. Biochem J. 369: 469-76. PMID: 12392447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cSHMT Antibody (A-2) | sc-365203 | 200 µg/ml | $322.00 | |||
cSHMT Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-529326 | 200 µg Ab; 10 µg BP | $361.00 | |||
cSHMT Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-522162 | 200 µg Ab, 40 µg BP | $361.00 | |||
cSHMT Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-543459 | 200 µg Ab; 20 µg BP | $361.00 | |||
cSHMT Antibody (A-2) AC | sc-365203 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cSHMT Antibody (A-2) HRP | sc-365203 HRP | 200 µg/ml | $322.00 | |||
cSHMT Antibody (A-2) FITC | sc-365203 FITC | 200 µg/ml | $336.00 | |||
cSHMT Antibody (A-2) PE | sc-365203 PE | 200 µg/ml | $349.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 488 | sc-365203 AF488 | 200 µg/ml | $364.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 546 | sc-365203 AF546 | 200 µg/ml | $364.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 594 | sc-365203 AF594 | 200 µg/ml | $364.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 647 | sc-365203 AF647 | 200 µg/ml | $364.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 680 | sc-365203 AF680 | 200 µg/ml | $364.00 | |||
cSHMT Antibody (A-2) Alexa Fluor® 790 | sc-365203 AF790 | 200 µg/ml | $364.00 | |||
cSHMT (A-2) Neutralizing Peptide | sc-365203 P | 100 µg/0.5 ml | $69.00 |