Cryopyrin Antikörper (6F12) Empfohlen für die Detektion von Cryopyrin aus der Spezies human per WB, IP und ELISA

Cryopyrin Antikörper (6F12): sc-134306

Cryopyrin Antikörper (6F12) wurde bewertet mit 3.1 von 5 von 19.
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Datenblatt

    • Anti-Cryopyrin Antikörper 6F12 ist ein monoklonales IgG2a κ Cryopyrin Antikörper, verwendet in 17 wissenschaftlichen Veröffentlichungen, in einer Menge von 100 µg/ml
    • raised against a recombinant protein corresponding to a region near the N-terminus of Cryopyrin of human origin
    • Empfohlen für die Detektion von Cryopyrin aus der Spezies human per WB, IP und ELISA
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom Cryopyrin (6F12): sc-134306 zu erhalten.

Is this antibody binding with mouse sample?

Gefragt von: bluehawk
Thank you for your question. We have not yet tested this antibody in mouse samples and so it is not a recommended species at this time. However, we are aware of some product citations using this antibody in mouse samples. For example, PMID: 25675528.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-06-15

Hi, we usually used Cryopyrin Antibody (H-66): sc-66846 for detecting Cryopyrin in mouse. You recommended Cryopyrin (6F12) : sc-134306 as a new alternative. But this antibody binds only in human. Have you checked whether it binds in mice?thanks

Gefragt von: suschu
Thank you for your question. We have not yet tested this mouse monoclonal antibody, Cryopyrin (6F12): sc-134306, for reactivity with mouse Cryopyrin so this species is not recommended at this time. However, the antibody is raised against a recombinant protein sequence that shares significant identity (88%) with the mouse protein, so there is a very strong chance that the antibody will cross-react with mouse. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a sample for testing. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-05-19

For Western Blot, is it recommended to use denatured or non-denatured conditions with Cryopyrin (6F12): sc-134306 antibody?

Gefragt von: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-03-27
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Rated 4 von 5 von aus worked well for western Used for western at highest concentration. detected protein.
Veröffentlichungsdatum: 2018-02-07
Rated 4 von 5 von aus Cryoprin Antibody (6F12) I tested this antibody in HCAEC lysates. I got the exact band but the the antibody didn't workout as much I expected. Gave me a little background in the PVDF membrane. May be it will work better for different cell line.
Veröffentlichungsdatum: 2018-01-24
Rated 1 von 5 von aus resultado inespecifico testé este anticuerpo para WB en tejido de conejo inflamado y el resultado no se reconoció banda al peso molecular estimado
Veröffentlichungsdatum: 2017-12-12
Rated 5 von 5 von aus 非常好! 这是本人用过众多NLRP3抗体中曝出条带最好的抗体,不是说其他品牌抗体不行,而是完全曝不出条带来,只有申请的这支抗体能曝出我想要的条带出来!非常棒!!!
Veröffentlichungsdatum: 2017-10-15
Rated 2 von 5 von aus sc-134306 antibody did not work at all in WB at 1:200/500/1000,so sorry
Veröffentlichungsdatum: 2017-09-17
Rated 3 von 5 von aus Does not work for fluorescence microscopy We were unable to use this Ab for IF in mouse BMDM
Veröffentlichungsdatum: 2017-09-05
Rated 3 von 5 von aus Works in human macrophages It works. However, several non-specific bands are really strong, even stronger than target one.
Veröffentlichungsdatum: 2017-08-23
Rated 2 von 5 von aus Failed to detect cyropyrin by WB in RAW264.7 . WB: No specific band. The dilution we used was 1:200
Veröffentlichungsdatum: 2017-08-13
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