COL10A1 Double Nickase Plasmid (h): sc-401960-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL10A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL10A1 Double Nickase Plasmid (h) and COL10A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL10A1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL10A1 Double Nickase Plasmid (h) | sc-401960-NIC | 20 µg | $410.00 | |||
COL10A1 Double Nickase Plasmid (h2) | sc-401960-NIC-2 | 20 µg | $410.00 |
COL10A1 encodes collagen type X alpha 1, a short-chain collagen that is selectively produced by hypertrophic chondrocytes and incorporated into the cartilage extracellular matrix during endochondral ossification. By shaping matrix composition and mineralization, COL10A1 contributes to growth plate maturation, chondrocyte differentiation programs, and extracellular matrix–receptor signaling that interfaces with pathways such as TGF-β/BMP and integrin-mediated mechanotransduction. Dysregulated COL10A1 expression or function is associated with abnormal skeletal development and has been reported as a marker of aberrant cartilage hypertrophy and remodeling in musculoskeletal disease contexts. In addition, COL10A1 expression patterns are used to interrogate hypertrophic cartilage states in developmental biology and tissue engineering models.
COL10A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL10A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL10A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL10A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL10A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.