CNTF Antibody (H-8): sc-365211

CNTF Antibody (H-8) is a mouse monoclonal IgG2a antibody that detects ciliary neurotrophic factor (CNTF) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CNTF is a crucial neuropoietic cytokine that promotes the survival and differentiation of various cell types, including sensory, sympathetic, and motor neurons, vital for maintaining nervous system integrity. CNTF (H-8) antibody targets proteins primarily located in the central nervous system and peripheral tissues, where CNTF exerts neuroprotective effects by binding to its receptor complex, including CNTFRα and the gp130/LIFRb heterodimer. This interaction activates downstream signaling pathways, particularly Stat1 and Stat3 transcription factors, leading to enhanced DNA synthesis and neuronal survival. CNTF has been implicated in amyotrophic lateral sclerosis (ALS), highlighting potential therapeutic applications. While mice lacking CNTF exhibit certain deficits, those with a homozygous null mutation in the CNTFRα gene experience severe motor neuron deficits and perinatal mortality, suggesting CNTFRα′s essential role in neonatal nervous system development and indicating the presence of additional CNTFR ligands critical during this developmental phase.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CNTF Antibody (H-8) References:

1. CNTF/LIF/gp130 receptor complex signaling maintains a VZ precursor differentiation gradient in the developing ventral forebrain.  |  Gregg, C. and Weiss, S. 2005. Development. 132: 565-78. PMID: 15634701
2. CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK.  |  Watt, MJ., et al. 2006. Nat Med. 12: 541-8. PMID: 16604088
3. Overexpression of CNTF in Mesenchymal Stem Cells reduces demyelination and induces clinical recovery in experimental autoimmune encephalomyelitis mice.  |  Lu, Z., et al. 2009. J Neuroimmunol. 206: 58-69. PMID: 19081144
4. CNTF induces regeneration of cone outer segments in a rat model of retinal degeneration.  |  Li, Y., et al. 2010. PLoS One. 5: e9495. PMID: 20209167
5. Ciliary neurotrophic factor (CNTF) promotes skeletal muscle progenitor cell (MPC) viability via the phosphatidylinositol 3-kinase-Akt pathway.  |  Hiatt, K., et al. 2014. J Tissue Eng Regen Med. 8: 963-8. PMID: 23147834
6. The cytokine ciliary neurotrophic factor (CNTF) activates hypothalamic urocortin-expressing neurons both in vitro and in vivo.  |  Purser, MJ., et al. 2013. PLoS One. 8: e61616. PMID: 23626705
7. TRPV1 on astrocytes rescues nigral dopamine neurons in Parkinson's disease via CNTF.  |  Nam, JH., et al. 2015. Brain. 138: 3610-22. PMID: 26490328
8. Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System.  |  Joly, S., et al. 2017. Neural Plast. 2017: 6818970. PMID: 29234527
9. Ciliary Neurotrophic Factor (CNTF) Protects Myocardial Cells from Oxygen Glucose Deprivation (OGD)/Re-Oxygenation via Activation of Akt-Nrf2 Signaling.  |  Zheng, K., et al. 2018. Cell Physiol Biochem. 51: 1852-1862. PMID: 30504707
10. Synergistic Protection of Retinal Ganglion Cells (RGCs) by SARM1 Inactivation with CNTF in a Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy.  |  Guo, Y., et al. 2024. Cells. 13: PMID: 38334594

The preferred CNTF antibody is CNTF Antibody (A-11): sc-25286.