Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CLK1 Double Nickase Plasmid (h): sc-402793-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLK1 Double Nickase Plasmid (h) and CLK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLK1 Antibody (F-12): sc-515897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLK1 Double Nickase Plasmid (h) | sc-402793-NIC | 20 µg | $410.00 | |||
CLK1 Double Nickase Plasmid (h2) | sc-402793-NIC-2 | 20 µg | $410.00 |
Human CLK1 (CDC-like kinase 1) is a dual-specificity kinase that phosphorylates serine/arginine-rich (SR) proteins and other splicing factors to regulate spliceosome dynamics and alternative pre-mRNA splicing. By modulating exon inclusion, RNA processing, and downstream transcript isoforms, CLK1 influences cell-cycle progression, stress responses, and transcriptional programs linked to proliferative signaling. CLK1 activity intersects with RNA metabolism pathways and post-transcriptional gene regulation networks that shape proteome diversity across tissues. Dysregulation of CLK1-mediated splicing control has been associated with aberrant isoform expression patterns observed in cancer biology and neurologic disease research contexts, supporting its use as a mechanistic node for studying splicing-dependent phenotypes.
CLK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.