Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CLIP-170 CRISPR/Cas9 KO Plasmid (m): sc-425158
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLIP-170 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CLIP-170 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLIP-170 Antibody (F-3): sc-28325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIP-170 CRISPR/Cas9 KO Plasmid (m) | sc-425158 | 20 µg | $397.00 |
Overview
Clip1 encodes CLIP-170, a microtubule plus-end–tracking protein that links dynamic microtubule tips to cortical sites and organelles to coordinate intracellular transport and cell architecture. CLIP-170 participates in regulation of microtubule polymerization, kinetochore–microtubule attachment during mitosis, and trafficking of vesicular and protein complexes via interactions with dynein/dynactin and other +TIP networks. Through these roles, Clip1 contributes to processes such as cell polarization, migration, and spindle organization that are frequently perturbed in proliferative and neurodevelopmental contexts. Altered microtubule dynamics and chromosome segregation fidelity associated with CLIP-170–dependent pathways are relevant to studying mechanisms linked to genomic instability and cytoskeletal dysfunction in disease models.
CLIP-170 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clip1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clip1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clip1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CLIP-170 protein expression.
This CRISPR knockout system enables efficient generation of Clip1-deficient cell models for investigation of CLIP-170 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clip1 exon(s) critical for CLIP-170 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clip1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CLIP-170 CRISPR/Cas9 KO Plasmid (m) and CLIP-170 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clip1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CLIP-170 HDR Plasmid (m) and CLIP-170 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clip1 homology arms to support homology-directed repair at defined Clip1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.