Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CDK11 CRISPR/Cas9 KO Plasmid (h): sc-405330
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDK11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDK11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk11 Antibody (8B6): sc-517026
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK11 CRISPR/Cas9 KO Plasmid (h) | sc-405330 | 20 µg | $397.00 |
Overview
CDK19 encodes a cyclin-dependent kinase that functions within the Mediator kinase module to modulate RNA polymerase II–dependent transcription and phosphorylation of transcription-associated substrates. Through regulation of transcription initiation and elongation programs, CDK19 influences cell-cycle progression, stress-responsive gene expression, and signal-dependent transcriptional networks linked to MAPK and other pathways. Dysregulated CDK19 activity or expression has been associated with altered proliferative control and transcriptional rewiring observed across multiple cancer contexts and other proliferative disorders. CDK11 is a multifunctional serine/threonine kinase implicated in cell-cycle regulation, pre-mRNA splicing, and transcriptional control, supporting studies of kinase network crosstalk and compensatory signaling in human cells.
CDK11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDK19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDK19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDK19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDK11 protein expression.
This CRISPR knockout system enables efficient generation of CDK19-deficient cell models for investigation of CDK11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDK19 exon(s) critical for CDK11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDK19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDK11 CRISPR/Cas9 KO Plasmid (h) and CDK11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDK19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDK11 HDR Plasmid (h) and CDK11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDK19 homology arms to support homology-directed repair at defined CDK19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.