Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD5 Double Nickase Plasmid (h): sc-402309-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD5 Double Nickase Plasmid (h) and CD5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD5 Antibody (UCH-T2): sc-1180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD5 Double Nickase Plasmid (h) | sc-402309-NIC | 20 µg | $410.00 | |||
CD5 Double Nickase Plasmid (h2) | sc-402309-NIC-2 | 20 µg | $410.00 |
CD5 is a type I transmembrane glycoprotein expressed primarily on T lymphocytes and a subset of B cells, where it functions as an immunomodulatory receptor that tunes antigen receptor signaling thresholds. Through interactions with components of the TCR/CD3 complex and associated phosphatases and adaptors, CD5 contributes to regulation of proximal tyrosine kinase cascades, calcium flux, and downstream transcriptional programs governing activation, anergy, and survival. CD5-mediated signaling influences thymocyte selection and peripheral tolerance, shaping immune homeostasis during inflammatory responses. Dysregulated CD5 expression or function has been linked to altered lymphocyte activation states in autoimmunity and to aberrant signaling networks in B cell malignancies, supporting its use as a marker and mechanistic node in immunology research.
CD5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.