CCDC8 CRISPR/Cas9 KO Plasmid (h): sc-406749
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCDC8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC8 CRISPR/Cas9 KO Plasmid (h) | sc-406749 | 20 µg | $397.00 |
Overview
CCDC8 (coiled-coil domain containing 8) encodes a cytoplasmic coiled-coil protein implicated in regulating cell growth and survival programs, with reported links to stress-responsive signaling and control of proliferative capacity. Functional studies suggest CCDC8 can influence apoptosis and checkpoint-related processes, connecting it to pathways that shape cellular homeostasis and transformation susceptibility. Altered CCDC8 expression or disruption has been explored in the context of tumor biology, where changes in growth regulation and survival signaling are common features. These attributes make CCDC8 a useful target for dissecting mechanisms that couple cytoskeletal or scaffold-like protein functions to signal transduction and cell fate decisions.
CCDC8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCDC8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCDC8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCDC8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCDC8 protein expression.
This CRISPR knockout system enables efficient generation of CCDC8-deficient cell models for investigation of CCDC8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCDC8 exon(s) critical for CCDC8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCDC8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCDC8 CRISPR/Cas9 KO Plasmid (h) and CCDC8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCDC8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCDC8 HDR Plasmid (h) and CCDC8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCDC8 homology arms to support homology-directed repair at defined CCDC8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.