Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CASZ1 Double Nickase Plasmid (h): sc-404756-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CASZ1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CASZ1 Double Nickase Plasmid (h) and CASZ1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CASZ1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CASZ1 Antibody (B-11): sc-398303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CASZ1 Double Nickase Plasmid (h) | sc-404756-NIC | 20 µg | $410.00 | |||
CASZ1 Double Nickase Plasmid (h2) | sc-404756-NIC-2 | 20 µg | $410.00 |
CASZ1 (castor zinc finger 1) encodes a nuclear zinc-finger transcription factor that regulates developmental gene-expression programs controlling cell fate decisions, differentiation, and tissue patterning. In human cells, CASZ1 influences transcriptional networks linked to cardiac and neurodevelopmental processes and can modulate pathways governing proliferation and lineage commitment. Altered CASZ1 expression or genomic disruption has been associated with neuroblastoma biology and other contexts where transcriptional control of growth and differentiation is perturbed. As a DNA-binding regulator, CASZ1 provides a tractable node for studying how cis-regulatory logic and transcription factor dosage shape normal and disease-relevant phenotypes.
CASZ1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CASZ1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CASZ1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CASZ1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CASZ1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.