calsyntenin-1 CRISPR/Cas9 KO Plasmid (m): sc-425768
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- calsyntenin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the calsyntenin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
calsyntenin-1 CRISPR/Cas9 KO Plasmid (m) | sc-425768 | 20 µg | $397.00 |
Overview
Clstn1 encodes calsyntenin-1, a type I membrane protein enriched in neurons that functions as a cargo adaptor linking vesicles to kinesin motors and coordinating anterograde transport in axons and dendrites. Through interactions with amyloid precursor protein (APP), synaptic components, and endosomal trafficking machinery, calsyntenin-1 contributes to synapse formation, receptor trafficking, and activity-dependent neuronal connectivity. Disruption of CLSTN1-dependent transport and synaptic homeostasis has been studied in the context of neurodevelopmental and neurodegenerative disease mechanisms, including pathways implicated in Alzheimer’s disease–related APP processing. In mouse systems, Clstn1 is frequently investigated to parse how long-range intracellular transport shapes circuit assembly, synaptic plasticity, and neuronal vulnerability.
calsyntenin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clstn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clstn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clstn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish calsyntenin-1 protein expression.
This CRISPR knockout system enables efficient generation of Clstn1-deficient cell models for investigation of calsyntenin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clstn1 exon(s) critical for calsyntenin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clstn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by calsyntenin-1 CRISPR/Cas9 KO Plasmid (m) and calsyntenin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clstn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by calsyntenin-1 HDR Plasmid (m) and calsyntenin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clstn1 homology arms to support homology-directed repair at defined Clstn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.