Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calregulin Double Nickase Plasmid (m): sc-419428-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calregulin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Calregulin Double Nickase Plasmid (m) and Calregulin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Calr. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calregulin Antibody (F-4): sc-373863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calregulin Double Nickase Plasmid (m) | sc-419428-NIC | 20 µg | $410.00 |
Mouse Calr encodes calregulin, a Ca2+-binding chaperone predominantly localized to the endoplasmic reticulum where it supports glycoprotein folding and quality control via the calnexin/calregulin cycle. By regulating ER Ca2+ homeostasis and interacting with nascent proteins, calregulin influences ER stress signaling, the unfolded protein response, and downstream proteostasis pathways. Calr also participates in cell adhesion and immune-relevant extracellular signaling when exposed on the cell surface, linking it to inflammation-associated processes. Dysregulated calregulin function has been associated with altered Ca2+ signaling, protein misfolding stress, and phenotypes relevant to oncogenesis and hematopoietic dysfunction in experimental systems.
Calregulin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Calr locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Calr. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Calr function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Calr-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.