Cacna2d2 CRISPR/Cas9 KO Plasmid (m): sc-425312
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cacna2d2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cacna2d2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cacna2d2 Antibody (G-5): sc-365911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cacna2d2 CRISPR/Cas9 KO Plasmid (m) | sc-425312 | 20 µg | $397.00 |
Overview
Cacna2d2 encodes the α2δ-2 auxiliary subunit of voltage-gated calcium channels, a key regulator of channel trafficking, membrane expression, and biophysical properties that shape calcium influx during neuronal excitability and synaptic transmission. By modulating CaV channel function, CACNA2D2 influences activity-dependent signaling, neurotransmitter release, and calcium-dependent gene expression programs that impact circuit development and plasticity. Genetic and functional alterations in α2δ subunits have been linked to neurodevelopmental and neurological phenotypes, with Cacna2d2 particularly associated with cerebellar function and motor coordination pathways. These features make Cacna2d2 a useful target for dissecting calcium-channel accessory mechanisms in neurobiology and electrophysiology-focused disease models.
Cacna2d2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cacna2d2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cacna2d2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cacna2d2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cacna2d2 protein expression.
This CRISPR knockout system enables efficient generation of Cacna2d2-deficient cell models for investigation of Cacna2d2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cacna2d2 exon(s) critical for Cacna2d2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cacna2d2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cacna2d2 CRISPR/Cas9 KO Plasmid (m) and Cacna2d2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cacna2d2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cacna2d2 HDR Plasmid (m) and Cacna2d2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cacna2d2 homology arms to support homology-directed repair at defined Cacna2d2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.