c-Fos Antikörper (D-1) Empfohlen für die Detektion von c-Fos aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA

c-Fos Antikörper (D-1): sc-8047

c-Fos Antikörper (D-1) wurde bewertet mit 3.9 von 5 von 18.
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Datenblatt

    • c-Fos Antikörper (D-1) ist ein Maus monoklonales IgG2b (kappa light chain) in einer Menge von 200 µg/ml
    • gezogen gegen Aminosäuren 210-335 gelegen am C-terminus von c-Fos aus der Spezies human
    • Anti-c-Fos Antikörper (D-1) ist empfohlen für die Detektion von c-Fos aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA
    • c-Fos Antikörper (D-1) ist erhältlich mit Alexa Fluor® 546 or Alexa Fluor® 594 für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 680 oder Alexa Fluor® 790 für WB (NIR), IF und FCM
    • erhältlich als TransCruz Reagenz für Gel Supershift und ChIP (sc-8047 X, 200 µg/0.1 ml)
    • Beachten Sie m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), unsere besonders zu empfehlenden rekombinanten Alternativen zu konventionellen sekundären anti-Maus IgG Reagenzien.
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom c-Fos (D-1): sc-8047 zu erhalten.

what is the best replacement for Sc-52

Gefragt von: ismaila
Thank you for your question. This is one of the most popular replacement antibodies for that now discontinued polyclonal antibody, sc-52. However, we offer several different options. Please contact our Technical Service Department for a recommendation based on your intended research application(s) and species reactivity.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-05-25

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct? 0 answers

Gefragt von: Emma Li
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-05-25

Can this antibody be used for the detection of c-fos in the macaque monkey. If not, which of your antibodies would you recommend?

Gefragt von: myprefrontal
Thank you for your question. We have not tested this antibody for reactivity with macaque, and are not aware of any publications citing use of the antibody in that species. We do offer an alternative antibody, c-Fos (6-2H-2F): sc-447, that has been published at least twice for detection of c-Fos in monkey. Please see PMID's: 9108029 and 11937718 for those references. A link to that product page on our website is below. https://www.scbt.com/scbt/product/c-fos-antibody-6-2h-2f
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-11-01

Can I still buy monoclonal antibodies from this company?

Gefragt von: KarenLindquist
Thank you for your question! Yes, you can certainly buy monoclonal antibodies from us! Please contact us at any time and we'd be glad to help you! You can reach us by phone at 800-457-3801 or by email at [email protected] or by live chat directly on our website, www.scbt.com.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2017-05-29

Could you please advise if this antibody is able to detect Fra proteins as is the case with the discontinued sc 253.

Gefragt von: Conor
Thank you for your question. The equivalent replacement mouse monoclonal antibodies to sc-253 are sc-166940: c-Fos (E-8) and sc-271243: c-Fos (C-10). Both of these mouse monoclonal antibodies are raised against the same peptide antigen used to generate the polyclonal antibody, sc-253. These recommended replacement mouse monoclonal antibodies are also recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and avian origin. Links to these products are provided below for your reference. https://www.scbt.com/scbt/product/c-fos-antibody-e-8 https://www.scbt.com/scbt/product/c-fos-antibody-c-10
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-01-19

I want to detect c-fos in my porcine samples. Will this antibody work?

Gefragt von: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Beantwortet von: TechService7
Veröffentlichungsdatum: 2016-12-23
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Rated 5 von 5 von aus - We examined the effects of a 3-week treatment of olanzapine (Olz) on c-Fos protein expression in stress-relevant brain sub/ regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation an animal model of depression.
Veröffentlichungsdatum: 2018-12-07
Rated 5 von 5 von aus 功能很齐全也很棒 我两个月前购买了这个产品,用它来做免疫荧光看的非常清楚,我想再买一盒接着做,同时我最满意的是它的性价比,很实惠也很实用,值得购买
Veröffentlichungsdatum: 2018-05-21
Rated 2 von 5 von aus It does not work well It did not work as well as other company's ab when I tested it by 1: 500.
Veröffentlichungsdatum: 2018-04-03
Rated 2 von 5 von aus No nuclear staining Level of expression appears comparable to other fos antibodies, but does not stain the the nucleus and non-specific binding is present at high concentrations.
Veröffentlichungsdatum: 2018-03-14
Rated 1 von 5 von aus Not satisfied! I tried a free sample of this antibody on free floating mouse brain sections and it didn't work. I used a concentration of 1:50 for the primary antibody, and 1:300 for secondary antibody (anti-mouse 488) and I didn't got any signal. Really not satisfied.
Veröffentlichungsdatum: 2018-02-23
Rated 5 von 5 von aus 功能很出色 我上一个月染得的fos,很出色,背景很清晰,很清晰。我上一个月染得的fos,很出色,背景很清晰,很清晰。
Veröffentlichungsdatum: 2018-02-05
Rated 1 von 5 von aus Disappointed I tried a sample of this antibody on my free-floating mouse brain sections which I have another set stained with my old, running out antibody to compare. It did not work at all at concentration ranging 1:100-2000.
Veröffentlichungsdatum: 2017-11-27
Rated 1 von 5 von aus Did not label cFos cells in sheep hypothalamus Using free-floating sections from sheep tissue, and an immunoperoxidase method of IHC, we did not see any immunostained cells using this antibody at dilutions of 1: 500, 1:1000, 1:2000.
Veröffentlichungsdatum: 2017-11-08
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