BMCP1 (UCP5) CRISPR/Cas9 KO Plasmid (h): sc-405051
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMCP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMCP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMCP1 (UCP5) CRISPR/Cas9 KO Plasmid (h) | sc-405051 | 20 µg | $397.00 |
Overview
SLC25A14 encodes BMCP1 (UCP5), a mitochondrial inner membrane carrier in the uncoupling protein family that modulates proton conductance and mitochondrial membrane potential, thereby influencing oxidative phosphorylation efficiency and cellular energy balance. UCP5 activity impacts mitochondrial reactive oxygen species generation, calcium handling, and susceptibility to stress-induced mitochondrial dysfunction, linking it to redox homeostasis and neuronal bioenergetics. Expression is enriched in nervous system contexts, and altered UCP5 regulation has been investigated in mechanisms relevant to neurodegeneration, metabolic stress, and mitochondrial disorders where coupling efficiency and oxidative stress are perturbed.
BMCP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC25A14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC25A14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC25A14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMCP1 protein expression.
This CRISPR knockout system enables efficient generation of SLC25A14-deficient cell models for investigation of BMCP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC25A14 exon(s) critical for BMCP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC25A14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMCP1 CRISPR/Cas9 KO Plasmid (h) and BMCP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC25A14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMCP1 HDR Plasmid (h) and BMCP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC25A14 homology arms to support homology-directed repair at defined SLC25A14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.