Biotin-XX hydrazide (CAS 211237-33-1)

Biotin-XX hydrazide, with the CAS number 211237-33-1, is a biotinylated derivative designed to facilitate the covalent attachment of biotin to various biomolecules via hydrazide chemistry. This compound is particularly valued for its extended spacer arm, which enhances its accessibility and reactivity when bound to target molecules. In biochemical research, Biotin-XX hydrazide is primarily used for the modification of aldehyde or ketone groups present on proteins, nucleic acids, and other biological molecules; this is achieved through the formation of stable hydrazone bonds. This specific functionality makes it an indispensable tool in the study of glycoproteins and other post-translationally modified structures where such reactive groups are often present. Researchers utilize this compound in applications such as affinity chromatography, where the strong and specific interaction between biotin and streptavidin is exploited to isolate and analyze modified compounds. Additionally, Biotin-XX hydrazide has been used in the preparation of biotinylated probes for various imaging and diagnostic assays, enabling the visualization and detection of specific biomolecular interactions and processes. The use of this chemical in such contexts provides critical insights into molecular function and dynamics, enhancing our understanding of complex biological systems without the need for invasive or high-impact techniques.