BF-1 CRISPR/Cas9 KO Plasmid (h): sc-402933
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BF-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BF-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BF-1 Antibody (D-12): sc-518188
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BF-1 CRISPR/Cas9 KO Plasmid (h) | sc-402933 | 20 µg | $397.00 |
Overview
Human FOXG1 encodes the forkhead box transcription factor BF-1, a master regulator of telencephalic patterning and neural progenitor maintenance during brain development. BF-1 acts primarily as a transcriptional repressor that integrates with developmental signaling networks, including Notch, Wnt/β-catenin, and TGF-β/BMP-associated programs, to control proliferation, differentiation, and regional identity. Through modulation of cell-cycle genes and neuronal fate determinants, FOXG1 influences cortical neurogenesis and synaptic maturation. Dysregulated FOXG1 activity or dosage is strongly associated with neurodevelopmental disorders and altered neuronal network function, making it a key target for mechanistic studies in neuronal lineage models.
BF-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOXG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOXG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOXG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BF-1 protein expression.
This CRISPR knockout system enables efficient generation of FOXG1-deficient cell models for investigation of BF-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOXG1 exon(s) critical for BF-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOXG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BF-1 CRISPR/Cas9 KO Plasmid (h) and BF-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOXG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BF-1 HDR Plasmid (h) and BF-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOXG1 homology arms to support homology-directed repair at defined FOXG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.