Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
basonuclin Double Nickase Plasmid (h): sc-405917-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- basonuclin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- basonuclin Double Nickase Plasmid (h) and basonuclin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BNC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: basonuclin Antibody (1F4): sc-517114
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
basonuclin Double Nickase Plasmid (h) | sc-405917-NIC | 20 µg | $410.00 | |||
basonuclin Double Nickase Plasmid (h2) | sc-405917-NIC-2 | 20 µg | $410.00 |
BNC1 encodes basonuclin, a zinc finger transcription factor enriched in stratified epithelia and germline-associated cell types, where it supports programs linked to proliferation and differentiation. Basonuclin localizes to the nucleus and has been implicated in regulation of ribosomal RNA transcription and broader gene expression networks that influence keratinocyte growth and tissue homeostasis. Through these transcriptional roles, BNC1 intersects with pathways controlling cell-cycle progression, chromatin state, and epithelial maturation. Altered BNC1 expression or epigenetic silencing has been reported in multiple tumor contexts, making it a useful locus for studying transcriptional dysregulation in cancer-related models.
basonuclin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BNC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BNC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BNC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BNC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.