ASPH Double Nickase Plasmid (h): sc-402938-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASPH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASPH Double Nickase Plasmid (h) and ASPH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ASPH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASPH Antibody (A-10): sc-271391
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASPH Double Nickase Plasmid (h) | sc-402938-NIC | 20 µg | $410.00 | |||
ASPH Double Nickase Plasmid (h2) | sc-402938-NIC-2 | 20 µg | $410.00 |
ASPH (aspartate β-hydroxylase) encodes an Fe(II)/2-oxoglutarate–dependent dioxygenase that hydroxylates aspartyl and asparaginyl residues within epidermal growth factor–like domains of select proteins. Through post-translational modification of extracellular and membrane-associated substrates, ASPH can influence protein–protein interactions and signaling processes involved in cell adhesion, migration, and differentiation, with reported links to Notch pathway modulation. Dysregulated ASPH expression has been observed across multiple tumor contexts and is frequently studied in relation to invasive growth and metastatic phenotypes. In addition to cancer biology, ASPH activity is investigated for its broader impact on secretory pathway protein maturation and cell-surface signaling networks.
ASPH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ASPH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ASPH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ASPH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ASPH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.