ANT1 Double Nickase Plasmid (h): sc-400679-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANT1 Double Nickase Plasmid (h) and ANT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC25A4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ANT1 Antibody (E-7): sc-518268
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANT1 Double Nickase Plasmid (h) | sc-400679-NIC | 20 µg | $410.00 | |||
ANT1 Double Nickase Plasmid (h2) | sc-400679-NIC-2 | 20 µg | $410.00 |
SLC25A4 encodes adenine nucleotide translocase 1 (ANT1), an inner mitochondrial membrane carrier that exchanges ADP and ATP across the mitochondrial membrane to couple oxidative phosphorylation with cytosolic energy demand. ANT1 is integral to mitochondrial bioenergetics, maintenance of membrane potential, and regulation of permeability transition and apoptosis, linking it to stress responses and mitochondrial quality control. Altered ANT1 function has been associated with neuromuscular and metabolic phenotypes, including mitochondrial myopathies and cardiomyopathy-related pathways, making it a key target for studying tissue-specific energy metabolism. In human cells, ANT1 also interfaces with broader mitochondrial carrier networks that shape redox balance and reactive oxygen species signaling.
ANT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC25A4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC25A4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC25A4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC25A4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.