ANO6 Double Nickase Plasmid (m): sc-430612-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANO6 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANO6 Double Nickase Plasmid (m) and ANO6 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ano6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANO6 Double Nickase Plasmid (m) | sc-430612-NIC | 20 µg | $410.00 | |||
ANO6 Double Nickase Plasmid (m2) | sc-430612-NIC-2 | 20 µg | $410.00 |
Mouse Ano6 encodes ANO6 (also known as TMEM16F), a Ca2+-activated phospholipid scramblase and ion channel that regulates plasma membrane lipid asymmetry and phosphatidylserine exposure. ANO6 activity integrates intracellular calcium signaling with membrane remodeling processes, including coagulation-related surface changes, vesicle shedding, and regulated cell death. In immune and hematopoietic contexts, ANO6 influences platelet procoagulant function and macrophage or lymphocyte membrane dynamics, linking it to pathways controlling hemostasis and inflammation. Dysregulation of ANO6-dependent scrambling has been associated with bleeding phenotypes and altered cell clearance, making Ano6 a useful target for studying membrane homeostasis and Ca2+-dependent signaling networks in mouse models.
ANO6 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ano6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ano6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ano6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ano6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.