ANO10 CRISPR/Cas9 KO Plasmid (h): sc-417456
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANO10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANO10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANO10 CRISPR/Cas9 KO Plasmid (h) | sc-417456 | 20 µg | $397.00 |
Overview
ANO10 (anoctamin 10/TMEM16K) is an endoplasmic reticulum–resident member of the TMEM16 family implicated in phospholipid scrambling and Ca2+-regulated membrane dynamics. It contributes to ER homeostasis, lipid distribution, and downstream processes including vesicle trafficking, organelle contact site function, and stress-response signaling pathways. Genetic disruption of ANO10 is associated with autosomal recessive spinocerebellar ataxia and related neurodegenerative phenotypes, supporting roles in neuronal maintenance and membrane lipid regulation. ANO10 is therefore studied in cellular models of neurobiology, ER stress, and lipid-mediated signaling.
ANO10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ANO10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ANO10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ANO10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANO10 protein expression.
This CRISPR knockout system enables efficient generation of ANO10-deficient cell models for investigation of ANO10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ANO10 exon(s) critical for ANO10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ANO10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANO10 CRISPR/Cas9 KO Plasmid (h) and ANO10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ANO10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANO10 HDR Plasmid (h) and ANO10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ANO10 homology arms to support homology-directed repair at defined ANO10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.