Annexin-2/ANXA2 Antibody (H-5): sc-48397

Annexin II Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1–50 of human Annexin II, also known as Annexin A2, Calpactin I heavy chain, Chromobindin-8, Lipocortin II, p36, and Placental Anticoagulant Protein IV. Annexin II is a calcium-dependent phospholipid-binding protein characterized by a conserved core domain composed of four annexin repeats, each forming a helix-loop-helix structure that enables binding to phospholipids in a Ca²⁺-dependent manner. This structural feature facilitates membrane-related processes such as endocytosis, exocytosis, and cellular adhesion. The unique N-terminal domain of Annexin II allows interaction with other proteins, notably forming a heterotetrameric complex with S100A10 (p11), known as calpactin I, which plays a significant role in signal transduction and cytoskeletal organization. By understanding the structure of Annexin II, researchers can better comprehend involvement in critical cellular functions like membrane trafficking and the regulation of fibrinolysis. Anti-Annexin II antibody (H-5) detects Annexin II of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), and is also reactive with additional species including canine and bovine. Targeting Annexin II with Annexin II monoclonal antibody (H-5) enables researchers to investigate its role in physiological processes and pathological conditions such as cancer progression, inflammation, and coagulation disorders. Available in both unconjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Annexin II (H-5) antibody offers versatility for a wide range of experimental applications.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Annexin-2/ANXA2 Antibody (H-5) References:

1. Annexin II overexpression correlates with stromal tenascin-C overexpression: a prognostic marker in colorectal carcinoma.  |  Emoto, K., et al. 2001. Cancer. 92: 1419-26. PMID: 11745218
2. Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration.  |  Liu, JW., et al. 2003. Oncogene. 22: 1475-85. PMID: 12629510
3. Annexin II-mediated plasmin generation activates TGF-beta3 during epithelial-mesenchymal transformation in the developing avian heart.  |  Krishnan, S., et al. 2004. Dev Biol. 265: 140-54. PMID: 14697359
4. Anti-annexin II antibodies in systemic autoimmune diseases and antiphospholipid syndrome.  |  Salle, V., et al. 2008. J Clin Immunol. 28: 291-7. PMID: 18322784
5. Annexin II/annexin II receptor axis regulates adhesion, migration, homing, and growth of prostate cancer.  |  Shiozawa, Y., et al. 2008. J Cell Biochem. 105: 370-80. PMID: 18636554
6. Annexin II promotes invasion and migration of human hepatocellular carcinoma cells in vitro via its interaction with HAb18G/CD147.  |  Zhao, P., et al. 2010. Cancer Sci. 101: 387-95. PMID: 20047591
7. Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.  |  Cinq-Frais, C., et al. 2015. Redox Biol. 4: 169-79. PMID: 25574848
8. Tenascin-c renders a proangiogenic phenotype in macrophage via annexin II.  |  Wang, Z., et al. 2018. J Cell Mol Med. 22: 429-438. PMID: 28857429
9. Expression of annexin II in gastric carcinoma and its role in gastric cancer metastasis.  |  Han, F., et al. 2017. World J Gastroenterol. 23: 7009-7015. PMID: 29097873
10. Annexin A2 and cancer: A systematic review.  |  Christensen, MV., et al. 2018. Int J Oncol. 52: 5-18. PMID: 29115416

The preferred Annexin-2/ANXA2 antibody is Annexin-2/ANXA2 Antibody (C-10): sc-28385.