Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ALP Double Nickase Plasmid (h): sc-401877-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ALP Double Nickase Plasmid (h) and ALP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDLIM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ALP Antibody (B-10): sc-365765
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALP Double Nickase Plasmid (h) | sc-401877-NIC | 20 µg | $410.00 | |||
ALP Double Nickase Plasmid (h2) | sc-401877-NIC-2 | 20 µg | $410.00 |
PDLIM3 encodes ALP (actinin-associated LIM protein), a PDZ–LIM domain scaffold enriched in striated muscle where it localizes to Z-discs and costameres. ALP couples α-actinin and other cytoskeletal components to signaling assemblies, supporting sarcomere organization, mechanotransduction, and actin cytoskeleton remodeling. Through its adaptor functions, ALP influences pathways governing muscle differentiation, contractile integrity, and stress responses. Altered PDLIM3 expression or variants have been associated with cardiomyopathy and myofibrillar defects, making it a relevant target for investigating muscle disease mechanisms.
ALP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDLIM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDLIM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDLIM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDLIM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.