Aldehyde dehydrogenase 3-A2/ALDH3A2 CRISPR/Cas9 KO Plasmid (h): sc-405313

Overview

Human ALDH3A2 (fatty aldehyde dehydrogenase, FALDH) is an NAD-dependent enzyme that oxidizes medium- and long-chain aliphatic aldehydes to their corresponding fatty acids, limiting the accumulation of reactive lipid aldehydes generated during lipid metabolism and oxidative stress. It is a key component of fatty alcohol and sphingolipid catabolism, including the degradation of fatty aldehydes derived from plasmalogen and ether lipid turnover. By controlling aldehyde burden, ALDH3A2 contributes to membrane lipid homeostasis and cellular redox balance. Loss-of-function variants are associated with Sjögren–Larsson syndrome and are studied in the context of neurocutaneous pathology and lipid-mediated cellular toxicity.

Aldehyde dehydrogenase 3-A2/ALDH3A2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ALDH3A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ALDH3A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ALDH3A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldehyde dehydrogenase 3-A2/ALDH3A2 protein expression.

This CRISPR knockout system enables efficient generation of ALDH3A2-deficient cell models for investigation of Aldehyde dehydrogenase 3-A2/ALDH3A2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ALDH3A2 exon(s) critical for Aldehyde dehydrogenase 3-A2/ALDH3A2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ALDH3A2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Aldehyde dehydrogenase 3-A2/ALDH3A2 CRISPR/Cas9 KO Plasmid (h) and Aldehyde dehydrogenase 3-A2/ALDH3A2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ALDH3A2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Aldehyde dehydrogenase 3-A2/ALDH3A2 HDR Plasmid (h) and Aldehyde dehydrogenase 3-A2/ALDH3A2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ALDH3A2 homology arms to support homology-directed repair at defined ALDH3A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.