ADX Reductase Antibody (E-2): sc-374436

ADX Reductase Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADX Reductase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADX Reductase antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADX Reductase, a crucial mitochondrial flavoprotein, plays a vital role in the electron transport chain by receiving electrons from NADPH, which is essential for the function of mitochondrial cytochromes P450. ADX Reductase facilitates cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-β hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. Alternate splicing of ADX Reductase results in two isoforms, and the human ADX Reductase gene is located on chromosome 17q24-q25, highlighting its significance in both genetic and metabolic contexts.

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ADX Reductase Antibody (E-2) References:

1. The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.  |  Ziegler, GA., et al. 1999. J Mol Biol. 289: 981-90. PMID: 10369776
2. Evidence for the cluster model of mitochondrial steroid hydroxylase system derived from dissociation constants of the complex between adrenodoxin reductase and adrenodoxin.  |  Hara, T., et al. 2000. Biochem Biophys Res Commun. 276: 210-5. PMID: 11006108
3. Adrenodoxin reductase homolog (Arh1p) of yeast mitochondria required for iron homeostasis.  |  Li, J., et al. 2001. J Biol Chem. 276: 1503-9. PMID: 11035018
4. Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.  |  Müller, JJ., et al. 2001. J Biol Chem. 276: 2786-9. PMID: 11053423
5. Phosphorylation of bovine adrenodoxin by protein kinase CK2 affects the interaction with its redox partner cytochrome P450scc (CYP11A1).  |  Bureik, M., et al. 2005. Biochemistry. 44: 3821-30. PMID: 15751958
6. Regional mapping of genes encoding human steroidogenic enzymes: P450scc to 15q23-q24, adrenodoxin to 11q22; adrenodoxin reductase to 17q24-q25; and P450c17 to 10q24-q25.  |  Sparkes, RS., et al. 1991. DNA Cell Biol. 10: 359-65. PMID: 1863359
7. Human adrenodoxin reductase: two mRNAs encoded by a single gene on chromosome 17cen----q25 are expressed in steroidogenic tissues.  |  Solish, SB., et al. 1988. Proc Natl Acad Sci U S A. 85: 7104-8. PMID: 2845396
8. Genetic obesity increases pancreatic expression of mitochondrial proteins which regulate cholesterol efflux in BRIN-BD11 insulinoma cells.  |  Caridis, AM., et al. 2019. Biosci Rep. 39: PMID: 30819824
9. The Escherichia coli FadR transcription factor: Too much of a good thing?  |  Cronan, JE. 2021. Mol Microbiol. 115: 1080-1085. PMID: 33283913
10. Fatty acid overproduction by gut commensal microbiota exacerbates obesity.  |  Takeuchi, T., et al. 2023. Cell Metab. 35: 361-375.e9. PMID: 36652945
11. A FadR-Type Regulator Activates the Biodegradation of Polycyclic Aromatic Hydrocarbons by Mediating Quorum Sensing in Croceicoccus naphthovorans Strain PQ-2.  |  Meng, Q., et al. 2023. Appl Environ Microbiol. 89: e0043323. PMID: 37098893
12. Competition for electron transfer between cytochromes P450scc and P45011 beta in rat adrenal mitochondria.  |  Yamazaki, T., et al. 1993. Mol Cell Endocrinol. 95: 1-11. PMID: 8243798

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ADX Reductase Antibody (E-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-ADX Reductase Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ADX Reductase Antibody (E-2) Specifications

Alternate Product Name: ADX Reductase

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 192-491

Epitope Target: Adrenodoxin Reductase

Epitope Species: human

Product Reactivity/Detection: ADX Reductase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for ADX Reductase Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin