ADAR1 Double Nickase Plasmid (h): sc-401611-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADAR1 Double Nickase Plasmid (h) and ADAR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADAR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADAR1 Antibody (D-8): sc-271854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAR1 Double Nickase Plasmid (h) | sc-401611-NIC | 20 µg | $410.00 | |||
ADAR1 Double Nickase Plasmid (h2) | sc-401611-NIC-2 | 20 µg | $410.00 |
ADAR encodes ADAR1, an interferon-inducible adenosine deaminase that catalyzes A-to-I RNA editing within double-stranded RNA structures, reshaping coding sequences, splicing, RNA stability, and miRNA targeting. By editing endogenous dsRNA, ADAR1 limits aberrant activation of innate immune sensors such as MDA5/MAVS and downstream type I interferon signaling, helping maintain self–non-self discrimination. ADAR1 also influences RNA processing in stress responses and can modulate translational outcomes through editing of repetitive elements and structured transcripts. Dysregulated ADAR1 activity or RNA-editing imbalance has been associated with interferonopathies, inflammatory phenotypes, and altered tumor–immune interactions, making it a key node for studying RNA surveillance and innate immune homeostasis.
ADAR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADAR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADAR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADAR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADAR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.