ACRV1 CRISPR/Cas9 KO Plasmid (m): sc-418963
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACRV1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACRV1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACRV1 Antibody (A-9): sc-398536
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACRV1 CRISPR/Cas9 KO Plasmid (m) | sc-418963 | 20 µg | $397.00 |
Overview
Acrv1 encodes acrosomal vesicle protein 1 (ACRV1), a testis-enriched component of the sperm acrosome that contributes to acrosome biogenesis and the molecular architecture required for fertilization. In mouse spermatids, ACRV1 supports late spermiogenesis processes including acrosome formation, sperm head shaping, and maturation events that influence zona pellucida interactions and the acrosome reaction. Dysregulation of acrosomal proteins is associated with reduced sperm function and male subfertility phenotypes, making Acrv1 a useful genetic entry point for studying reproductive biology. This gene is frequently leveraged to dissect germ cell differentiation programs and acrosome-related pathways that underlie sperm quality and fertilization competence.
ACRV1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acrv1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acrv1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acrv1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACRV1 protein expression.
This CRISPR knockout system enables efficient generation of Acrv1-deficient cell models for investigation of ACRV1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acrv1 exon(s) critical for ACRV1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acrv1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACRV1 CRISPR/Cas9 KO Plasmid (m) and ACRV1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acrv1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACRV1 HDR Plasmid (m) and ACRV1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acrv1 homology arms to support homology-directed repair at defined Acrv1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.