Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACE CRISPR/Cas9 KO Plasmid (h): sc-400759
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACE CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACE Antibody (2E2): sc-23908
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACE CRISPR/Cas9 KO Plasmid (h) | sc-400759 | 20 µg | $397.00 |
Overview
Angiotensin-converting enzyme (ACE) is a zinc-dependent dipeptidyl carboxypeptidase that catalyzes conversion of angiotensin I to the vasoactive peptide angiotensin II and degrades bradykinin, positioning it as a central regulator of the renin–angiotensin system. Through modulation of angiotensin II/AT1R signaling, ACE influences vasomotor tone, sodium and fluid balance, oxidative stress, and inflammatory programs that intersect with MAPK and NF-κB-linked responses in vascular and renal tissues. ACE activity contributes to endothelial function and extracellular peptide homeostasis, and altered ACE expression or variants are frequently studied in cardiovascular and renal disease biology. These functions make ACE a useful node for investigating blood pressure regulation, vascular remodeling, and inflammation-associated tissue remodeling in human cell models.
ACE CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ACE gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ACE together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ACE open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACE protein expression.
This CRISPR knockout system enables efficient generation of ACE-deficient cell models for investigation of ACE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ACE exon(s) critical for ACE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ACE genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACE CRISPR/Cas9 KO Plasmid (h) and ACE CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ACE locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACE HDR Plasmid (h) and ACE HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ACE homology arms to support homology-directed repair at defined ACE target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.