Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACCα Double Nickase Plasmid (h): sc-401102-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACCα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACCα Double Nickase Plasmid (h) and ACCα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACACA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACCα Antibody (D-5): sc-137104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACCα Double Nickase Plasmid (h) | sc-401102-NIC | 20 µg | $410.00 | |||
ACCα Double Nickase Plasmid (h2) | sc-401102-NIC-2 | 20 µg | $410.00 |
ACACA encodes acetyl‑CoA carboxylase alpha (ACCα), a biotin-dependent enzyme that catalyzes the ATP-dependent conversion of acetyl‑CoA to malonyl‑CoA, the committed step in de novo fatty acid synthesis. By controlling malonyl‑CoA levels, ACCα coordinates lipid anabolic flux and indirectly regulates mitochondrial fatty acid β‑oxidation via inhibition of CPT1, linking nutrient status to energy homeostasis. ACCα activity is integrated with AMPK signaling and lipid-responsive transcriptional programs, shaping membrane biogenesis, lipid droplet formation, and acetyl‑CoA partitioning. Dysregulated ACACA expression or activity has been associated with metabolic disease phenotypes and lipid-dependent vulnerabilities in cancer cell metabolism, supporting its use in studies of metabolic rewiring and lipogenesis.
ACCα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACACA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACACA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACACA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACACA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.